Bioinformatics Module – Metagenomics annotathon Assignment Report Template Writing Assignment Help. Bioinformatics Module – Metagenomics annotathon Assignment Report Template Writing Assignment Help.
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Please use the attached template and sequence assigned to me. Answer all the question from section 1 to 4
Section 1.Finding the Open Reading Frame (ORF)
a.Genomic Sequence:
b. Translation Used:
c.Translated Sequence
d.Commentary on Translation
Section 2.BLAST Data and Sequence Alignment
a.Summary of BLAST Data (Maximum 2 pages of figures and text)
Be selective about what you show here – it might be worth summarizing your BLAST output as a table.Think about what information might be relevant to a reader.There is no point in having 10 pages of raw data showing pair-wise alignments.It would be better to find the closest homologues and provide a multiple alignment (see part b) with your sequence against their full sequences.
You will need to follow the Entrez links from your BLAST output to get these sequences in FASTA format and perform a multiple alignment. Show the minimum number of alignments to the nearest homologues to demonstrate what your protein relates to.
It is possible some of your top hits in BLAST may be the same protein from the same species (just sequenced by different researchers or in different context (for example, as synthetic constructs).Be wary about this as it is far more informative to be selective and show an alignment with proteins from different species if possible.
b.Multiple Sequence Alignment
Alignments are useful if you can align your sequence against the full-length versions of the homologues.Click on the accession numbers in your BLAST output data to full sequences and other information regarding the homologues. Highlight any regions of high homology.
c.Commentary on the BLAST data and Alignment (Maximum 100 words)
How confident are you about the BLAST data – remember to quote relevant scores and E-values.Has this protein ever been sequenced before? What is the percentage identity to the nearest homologues?If your putative protein is incomplete can you predict how much might be missing by examining the sequences of the homologues?
Section 3.Investigating Conserved Domains
- Protein domains and structure (Maximum 2 pages of figures and text)
This section will need to be completed with judicial use of supporting figures and text.Indicate which websites/databases were used to make any predictions
.
Can you infer anything about conserved functional domains of your putative protein either directly by analysing the primary sequence and/or by comparison to related proteins?Does your protein belong to any families or super-families of proteins based on conserved domains?
What is known about the structure? If you have a complete protein what is the MW and isoelectric point?If your protein is incomplete what are the values for the nearest homologue?If your protein is incomplete could we predict what is missing based on the sequence of nearest homologues? Is there a cellular localization signal – can you predict where in the cell this protein belongs? (Be careful about localization if it is likely prokaryotic!)
Optional – Assuming its structure has not been resolved – what can we infer about the 3D structure of your putative protein if anything? Note – remember the primary sequence database is many orders of magnitude larger than the tertiary structure database so this may not be applicable for all putative proteins.
Section 4.Proposed Biological Function and Taxonomy
a.Proposed Biological Function (Maximum 500 words plus supporting figures)
What does your putative protein do? When your ORF’s homologues have known functions, or if the ORF presents known conserved domains you may be able to predict a possible biological function. What can you conclude from the BLAST analysis and any conserved functional domains? You will need to search the relevant literature regarding these domains or the homologues themselves.
b.Phylogenetics
You may need to construct a basic phylogenetic tree using your BLAST output data. What can you infer about the species of origin of your DNA?What data does your phylogenetic tree provide? Does it make sense – would this class of organism likely to be found in the sample pool?
c.Assigning a Gene Ontogeny (GO) Term
Assign a relevant GO term from the simplified list of GO terms on the LMS from either (or both if applicable) the Molecular Processes or Biological Processes list.
References
Papers cited
Note: List the URLs of online tools used either in the figure captions or in text when referring to the relevant data.
A Note on General Scholarship
This report involves being judicious about what data you show.Remember that you are making a case for a proposed function and origin for your putative protein. You need to show the relevant data that makes this case for you.
Remember to include figure captions and legends and include references. Avoid simply cutting and pasting data from websites.
You will need to work on your figures to highlight regions/sequences of interest and to add labels which increase the clarity of your points. For example, highlight the coding portion of your genomic DNA or all I will see is a nucleotide sequence with no context.
Bioinformatics Module – Metagenomics annotathon Assignment Report Template Writing Assignment Help[supanova_question]
Individual project? Business Finance Assignment Help
Davy Jones needs to file taxes for his business and provides the following information.
Using your Tax Guide, you determine you’re going to be required to prepare a 1040 Schedule C Profit or Loss form a Business and 1040 Schedule SE Self Employment Tax for Jones’ Sole Proprietorship.
Davy Jones’ Nautical Tours is owned and operated as a Sole proprietorship by David K. Jones (SSN 000-00-0000)
Principal Business or Professional Activity Code; 114110
Principal business; Charter fishing tours
Employer Identification Number 867530950
Business address
1008 North Harbor Drive
San Diego, CA 92103
Home address
8700 Lake Murray Blvd #2
San Diego, 92119
- The cash accounting method is utilized
- Jones did materially participate in the operation of the business
- The business started January 1st, 2012
Wages consist of $25,000 paid to one employee and $15,000 to another
Depreciation expense; Jones owns a fishing boat, The Jolly Roger, which he purchased for $850,000. The depreciation on the income statement is book depreciation and will differ from what is actually used on Line 13 Depreciation of form 1040 Schedule C.
The boat is depreciated using the Modified Accelerated Cost Recovery System (MACRS), half year convention, is in its 4th year of service and the 10-year recovery period is used.
Utilize Publication 946 How to Depreciate Property to calculate the correct depreciation amount to enter on the 1040 Schedule C. Locate Table A1 in the Appendix to calculate the tax-deductible depreciation.
Interest expense is interest paid on the loan for the boat
Insurance payments were made to cover liability for guests and for the boat
Payroll taxes; Federal Insurance Contributions Act (FICA); Social Security and Medicare payments made on the behalf of employees
Federal Insurance Contributions Act (FUTA) made on behalf of employees
Travel was purely for business purposes
Parking fines are tickets Jones received for parking illegally
Lease expense was paid to Honda Motors for his mother’s car lease, who is not involved in the business in any way
Davy Jones’ Nautical Tours 2016 Income Statement
|
Revenue |
$ 400,950.00 |
|
Wages |
$ 40,000.00 |
|
FICA |
$ 3,060.00 |
|
FUTA |
$ 85.00 |
|
Depreciation |
$ 85,000.00 |
|
Interest |
$ 43,900.00 |
|
Advertising |
$ 6,350.00 |
|
Supplies |
$ 23,795.00 |
|
Legal fees |
$ 6,940.00 |
|
Insurance |
$ 26,700.00 |
|
Boat slip rental |
$ 33,010.00 |
|
Travel |
$ 6,250.00 |
|
Office expense |
$ 7,940.00 |
|
Parking fines |
$ 1,350.00 |
|
Lease expense |
$ 3,650.00 |
|
Net Profit |
$ 112,920.00 |
Assignment Guidelines
To prepare the Sole Proprietorship’s tax return, complete the following steps:
- Ensure that you have Adobe Reader installed on your computer to open and use the downloadable IRS forms.
- Go to the IRS Web site and download the fill-in tax forms that you will need to complete.
- Complete all necessary downloaded fill-in forms on the computer and save.
- Save the completed forms as PDFs and upload for grading.
- You may not utilize Tax preparation software to complete this assignment! This constitutes cheating!
- Save the completed forms as PDFs and upload for grading; double check your PDFs to ensure data was retained when they were uploaded to Submitted Assignments; blank forms will yield 0 points!
You will be required to fill out and submit the following forms:
- 1040 Schedule C – Profit or Loss from Business (Sole Proprietorship)
- 1040 Schedule SE – Self Employment Tax
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breast cancer diagnosis and individualized treatment different subtype of breast cancer Writing Assignment Help
please ONLY use and paraphrase articles/material i provided. DO NOT use other outside resources. thank you
pathophysiology of breast cancer discuss the latest developments and studies.
In addition to different treatment plans, please include advancements in diagnostic tests to differentiate subtypes.
In the conclusion section, please share your experience (if you have any) and include some tips for your nurse practitioner colleagues.
You can focus on the relationship between pathophysiology and evidence-based medicine and prepare a case study/literature review. As you know, “literature review” means the process of reading, analyzing, evaluating, and summarizing scholarly materials about a specific topic
Your paper should be MINIMUM 3000 words long, not counting citations or pictures. This is the minimum number and please keep in mind that the more is better.
-Please follow APA style and include at least 10 peer-reviewed references
please use only use and paraphrase articles/material i provided. DO NOT use other outside resources.
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University of Michigan Democratic Backsliding in the United States Paper Writing Assignment Help
Write a 3 to 5 page double-spaced (1” margins, 12 point font) “reflection paper” that answers the following questions:
Some analysts worry that the U.S. may be in danger of backsliding from a democracy to a non-democratic regime. To what extent do you agree or disagree with this assessment? In your answer, analyze the democratic backsliding that has occurred in other countries (e.g., Nicaragua, Venezuela, Turkey, Hungary, Poland, etc.). What types of actions were taken by leaders of those countries that produced the democratic backsliding? To what degree have these types of actions occurred in the U.S., and what indications are there that they could occur in the near future? Are there other conditions that might influence the prospects for democratic backsliding? Finally, what do these case comparisons suggest are the main factors protecting democracy from backsliding?
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Final Project Using Stock Data Collected During the Semester Business Finance Assignment Help
Here is the instructions for the final stock tracker spreadsheet. The questions have several links that will show you how to do the calculations. Use the 25th as the last date to track your stocks. As you will see when you look at the assignment, there are several parts of this that you can go ahead and set up formulas for, and then when you put in the last day’s prices you will be mostly done
Once you’re done, you can simply upload the file to the assignment box.
The first file is the job requirement. The second and third files are the homework I started in the semester. Please continue this and complete it.
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Final report Writing Assignment Help
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Integrative Report (30% final grade) Complete an analysis and research into the market and consumer behaviour of your chosen product by producing a report (roughly 10 pages or 3000 words) reflecting key concepts covered in the course. Provide suggestions or recommendation on strategies that should be focused on in the development of any future marketing programs. The report should be created as if the student is a marketing consultant advising the marketing and product managers of a client firm. 75 |
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Reflective Summary (10% final grade) A summary of online discussions and journalling (3 to 4 pages, roughly 1000 words). 25 |
Final report Writing Assignment Help[supanova_question]
I need the questions to be answered separately Writing Assignment Help
Here are The questions I need answers for :
B. Explain why land, culture, and history are important to American Indian identity. Provide at least two examples (one must be from a class reading). (15 points)
C. Explain the genesis of the term “Indian” and how it has contributed to the persistence of stereotypes of Indigenous people today.Provide at least two examples in your explanation. (20 points)
Essay Question (5-6 paragraphs): 40 points
E. Explain the Marshall Trilogy and how it is foundational to Federal Indian Policy. Briefly explain all three-court cases with dates and how they have impacted the relationship between the U.S. Federal government, States, and Federally Recognized Tribes.
Here I have the concepts:
Concepts
Be able to discuss the historical conception of the term Indian with other terms/imagesthat were used by colonizers to identify Indigenous peoples. Berkhofer’s “The White Man’s Indian” and Class Presentation, “Whats in a name? “
Know the 3 underlying assumptions by the US Government of Indian policy?
Be familiar with the Timeline from the AIS History Presentation and be able to briefly explain the time periods of “Treaty Making Era, Removal Era, Allotment Era”, and the “Reservation Era”.
Know how many federally recognized tribes in the US? How many in Arizona?
Peoplehood: Be able to identify and describe the four concepts that make up Peoplehood?
History and Conflict- Describe how telling the history of the American Indian Experience has been traditionally misrepresented, provide examples from information presented in class.
Be able to briefly explain what the Marshall trilogy is and how it impacted Indigenous tribes’ relationship with the state and federal government. (Johnson v. M’Intosh, 1823; Cherokee Nation v. Georgia, 1831, Worcester v. Georgia, 1832)
Be able to identify and explain sovereign rights American Indian tribes possess in contemporary society.
Also, I have uploaded 2 files to help, Please ask me if you have any questions!
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Write a paper on a physical security incident and recommend fixes to prevent the incident from happening again Writing Assignment Help
Write a paper on a physical security incident and recommend fixes to prevent the incident from happening again
Due date is July 21, 2019 at 11:59pm EST
- Research physical security incidents and write a paper on one which interests you the most. Also recommend controls to prevent the incident from happening again.
- Paper MUST be submitted in APA format
- Propose a possible change in that organization’s physical security that could have prevented the breach/failure for that scenario
- Submit at least 2 but no more than 4 pages double spaced
- No photos or graphs
- Reference all sources used in APA format
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Click Create Journal Entry above to get started. Business Finance Assignment Help
Click Create Journal Entry above to get started.
Please note that this journal assignment is based on a pretend scenario and fictitious money. However, the assignment is based on actual stock pricing in real time situations. Do not invest your personal money for this assignment.
To assist you with your Journal entry please use the following template by clicking here.
The capital markets and the ability to raise funds for corporate uses are essential to the U.S. economic systems. For this assignment, imagine that you have $25,000 to invest in U.S. companies. You are buying used stock. The company got the money when it issued the stock originally. You will be buying it from an existing owner.
You are investing, or buying the stock, because you believe the company will make money and pay you a dividend in cash. Each share of stock that you buy entitles you to any dividend declared and a vote at the annual stockholders’ meeting.
The stock also allows you the ability to earn your money back by selling the stock. Of course, investing in stocks is risky and there is the possibility that the stock you buy will be worth less when you want your money back. The company is not obligated to give you any of your money back. You will only get your money back if another investor wants to buy your stock.
For your first journal entry complete the following:
- Indicate the companies you are investing in: Please use your knowledge and experience and select three (3) US companies that are publicly traded. Make sure you are practicing good diversification. Jim Cramer, Money Manager, on CNBC, plays a game at the end of his show called “Am I Diversified.” Check out a short clip to get a sense of industry diversification at https://www.youtube.com/watch?v=f3lDxexupcE.
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Discussion Questions Mathematics Assignment Help
Respond to the following questions in a minimum of 175 words each:
1.Review the example provided for the panel data in Figure 10.5 and post your thoughts on the application of the dependent sample tests in your business context (example in attachment)
2.
Imagine that you have conducted a two-sample test and determined that the difference was not statistically significant. While one mean was 4.3 and the other was 3.9, the p level for the t test was p=.07. Your management team says, “Well, the difference may not be statistically significant, but the difference is there! Discuss how you would respond and how you would explain the purpose of the t test and significance in this case.
3.The ANOVA table contains the output of the ANOVA calculations. Review example given on pp 379 and Figure 11.6 in Chapter 11 illustrating the Excel ANOVA table. Post your questions, insights and takeaways on the use of the ANOVA table. (example is in second attachment)
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