Testing the efficiency of SIGS-mediated RNAi against M. Persicae

3 experiments were conducted and methods are written up. Experiments conducted were to assess whether spraying dsRNA can silence a target gene and affect aphid movement and reproduction.

Experiment 1: aphids sprayed while on plants.

Experiment 2: aphids feeding on sprayed plants.

Experiment 3: aphids alone were sprayed and then transferred to the plant to feed for 14 days. One aphid life cycle is completed in 14 days, so reproduction was assessed after 14 day period.

Nanoparticles were bound to dsRNA to see if it would improve the delivery to the plants and aphids, and improve the efficacy of treatments.

To summarise the results, there was a stronger effect on movement than reproduction in all tested conditions. No improved effect observed with nanoparticle-bound dsRNA.

I have attached a draft (which contains all sections) with feedback to help, a lot may seem repetitive. Please re-write concisely and professionally. Need help with statistical analysis, may need to be re-done (Fisher’s exact/chi squared test when comparing number of aphids for reproduction or number of dead aphids etc. highlighted in the document). Please do stats analysis where required even if I have not analysed.

Please note that I’ve attached an excel file with all the data and one-way ANOVA and t-test results. Post-hoc Tukey’s test results were done on an online platform, not present here, might need to be re-done.

Graphs already done. I will edit that myself, please use that for reference when writing results. Some graphs may present the same results in different ways (e.g. 4.11 and 4.12). However, please note comment on figure 4.10. Wouldn’t mind some guidance/ideas with compiling graphs 4.8 to 4.10 together.

I will be requesting an additional chapter (final discussion for the whole of the thesis) to be completed after this order has been done, would like for the same writer to work on it, thank you.

Thank you so much.

UPD: Cl will place order for another chapter which is related to that paper tomorrow. But it can be in progress when this order is completed. So, the same wr should be assigned and next order should be put on hold until this one is completed.

Lab Report 1 Essay

this is a lab report which contains three 2 previous assignments that we have already done ( II will upload these 2 assignments) and for the new one, I will provide everything that you need for this lab report. There is 3 lab report and I need to combine them into 1 lab report.
There is a link that I want you to use as one of the references. So to make sure so there is module 1 part 1, 2, and 3. I already have 1 and 2 I need part 3 which will include 1, 2, and 3.

I need your confirmation for your help regarding my Phd thesis. It is an experiment comparing the RNA seq

I need your confirmation for your help regarding my Phd thesis. It is an experiment comparing the RNA seq of two treatments. The first for growth of Dunaliella cells in inorganic autotrophic medium, the second is the same cells growing in mixture of organic and inorganic mixotrophic medium. The RNA seq data are already analyzed, and I have the resulting heat map and Gene ontologies and a combined transcriiptome assembly for the used 6 replicates (3 replicates for each treatment of the 2 treatments). I have no experience to guide with detailed instructions. I needed abstract, introduction, materials and methods, results, and discussion for the thesis. It needs no software experience but I think you may need use user friendly bioinformatics websites to help you to find interpretations and detect the pathways that are up or down regulated in response to the different conditions of growth for the cells. I also may need to understand some information that you will provide me on your written manuscriipt as lt is my first RNAseq experiment.

review lecture and answer questions

review lecture and answer questions. review lecture and answer questions. lecture: https://learn-us-east-1-prod-fleet02-xythos.conten…
questions :
1) What does the “Sixth Great Extinction” mean? What is the cause of this event and what can be done to stop it?
2) Name the two primary Greenhouse Gasses.
3) Name five negative consequences of increased Greenhouse emissions.
4) Examine the Mauna Loa CO2 concentration graph in this week’s PPT presentation. How much has the CO2 level increased increased between the years 1800 and 2000? (You can write your answer in the form ‘from _____ ppm to _____ ppm.’)
5) Examine the average yearly temperature graph from the Blue Hills Observatory. How much has the yearly temperature in Milton, Massachusetts increased on average over the past 200 years?
6) Describe what ‘Ocean Acidification’ is. Be sure to name the specific gaseous emission that causes ocean acidification. Be sure to describe the effect ocean acidification will have on sea life that have calcium carbonate shells.
7) What is the normal pH of ocean water away from the coasts? Anything with a pH above 7.0 is considered basic, and anything with a pH below 7.0 is considered acidic. Does the water actually have to become acidic to have a negative effect on life in the oceans?
8) Do you think that humans have exceeded the Earth’s ‘Carrying Capacity’? Note: I am asking for your opinion, which means the only incorrect answer is no answer at all.
9)Name five specific actions that you can personally take to lower your environmental impact on the planet Earth.

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review lecture and answer questions

review lecture and answer questions

review lecture and answer questions

review lecture and answer questions. review lecture and answer questions. lecture : https://learn-us-east-1-prod-fleet02-xythos.conten…
questions:
1) What does the “Sixth Great Extinction” mean? What is the cause of this event and what can be done to stop it?
2) Name the two primary Greenhouse Gasses.
3) Name five negative consequences of increased Greenhouse emissions.
4) Examine the Mauna Loa CO2 concentration graph in this week’s PPT presentation. How much has the CO2 level increased increased between the years 1800 and 2000? (You can write your answer in the form ‘from _____ ppm to _____ ppm.’)
5) Examine the average yearly temperature graph from the Blue Hills Observatory. How much has the yearly temperature in Milton, Massachusetts increased on average over the past 200 years?
6) Describe what ‘Ocean Acidification’ is. Be sure to name the specific gaseous emission that causes ocean acidification. Be sure to describe the effect ocean acidification will have on sea life that have calcium carbonate shells.
7) What is the normal pH of ocean water away from the coasts? Anything with a pH above 7.0 is considered basic, and anything with a pH below 7.0 is considered acidic. Does the water actually have to become acidic to have a negative effect on life in the oceans?
8) Do you think that humans have exceeded the Earth’s ‘Carrying Capacity’? Note: I am asking for your opinion, which means the only incorrect answer is no answer at all.
9) Name five specific actions that you can personally take to lower your environmental impact on the planet Earth.

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7 mins ago

review lecture and answer questions

review lecture and answer questions

Lab : about fossil hominins based on the analysis of qualitative and quantitative empirical data

Lab : about fossil hominins based on the analysis of qualitative and quantitative empirical data. Lab : about fossil hominins based on the analysis of qualitative and quantitative empirical data. The purpose of this lab is for you to infer appropriate conclusions about fossil hominins based on the analysis of qualitative and quantitative empirical data and identify key physical (morphological), physiological (functional), and behavioral traits in human fossil ancestors.
you become familiar with the following important content knowledge in anthropology:
later Homo and Homo sapiens evolution
hypotheses to explain the origins of modern humans
infer specific behaviors based on anatomical traits
identify anatomical similarities and differences in the later members of the genus Homo
identify anatomical traits used to identify modern Homo sapiens in the paleoanthropological record
Print the Lab 12 worksheet and write-in your answers OR open the lab in Adobe (or whatever pdf. reader you use) and type-in your answers using the text-box function
Step 2: Open the Lab 12 workstations.
Kabwe, Zambia (Africa), ~275-325 kya
Homo heidlebergensis by Geospatial and Virtual Archaeology Lab and Studio on Sketchfab
Steinheim, Germany, 250-350 kya

Homo heidelbergensis (Steinheim skull)(1979rp45) by RLA Archaeology on Sketchfab

Florisbad, Orange Free State, South Africa, 250-350 kya

Homo heidelbergensis (Florisbad) (1979rp24) by RLA Archaeology on Sketchfab

Workstation 2: The NeanderthalsInstructions:
Observe the 3D models of the Neanderthals below.
Compare and contrast the two crania. How are they similar? How are they different? Record your response on the Lab 12 worksheet.
Identify three observable traits that are present on both crania that are derived traits in Neanderthals. Record your response on the Lab 12 worksheet.
La Chapelle-aux-Saints, France, 47-56 kya
(433rp7) by RLA Archaeology on Sketchfab
Gibraltar 1, ~35 kya, found in Forbes Quarry, Gibraltar (one of the youngest Neanderthal fossils, ~35 kya is super recent!!)
Gibraltar 1 Skull with Texture by Wellesley College on Sketchfab
Workstation 3: Neanderthals vs. Modern Homo sapiensInstructions:
Observe the 3D models of mandibles from a Neanderthal and a modern Homo sapiens below.
Compare and contrast the two samples. How are they similar? How are they different? Record your response on the Lab 12 worksheet.
Identify one observable trait that is present on the Neanderthal mandible, but not present on the modern Homo sapiens mandible. Record your response on the Lab 12 worksheet.
Identify one observable trait that is present on the modern Homo sapiens mandible, but not present on the Neanderthal mandible. Record your response on the Lab 12 worksheet.
Spy Cave, Namur, Belgium, ~40 kya (Neanderthal)

(Spy 2) Mandible (433rp20) by RLA Archaeology on Sketchfab

Hotu Cave, Iran, ~9,900 – 7,200 years before present (modern Homo sapiens)

Homo sapiens (Hotu Cave) (1979rp28) – mandible by RLA Archaeology on Sketchfab
Workstation 4: Evolution of Homo sapiensAs with other transitions in hominin evolution, modern traits do not appear fully formed or all at once. The traits common to modern Homo sapiens appeared in a mosaic manner. In this workstation, you will look at how our skull anatomy has changed over time and you will also observe geographic variation in our species.
Instructions:
Observe the 3D models of modern Homo sapiens below. (Some of the models are a little dark. Just do the best you can.)
Describe how the cranial morphology in modern Homo sapiens has evolved (changed) over time based on your observations of the samples below. Record your response on the Lab 12 worksheet.
Identify one observable derived modern Homo sapiens trait that all the crania share. Record your response on the Lab 12 worksheet.
Which hypothesis to explain the origins of modern Homo sapiens is best supported by the morphology of these crania (Multiregional or Out of Africa)? Please be cognizant of the times and geographical areas represented by the samples. Please explain your answer and any limitations associated with the data (aside from the small sample size). Record your response on the Lab 12 worksheet.
Herto: Middle Awash, Ethiopia, ~160 kya
Homo sapiens Cranium by UCFanthropology on Sketchfab
Skhul 5 sample: Mugharet es-Skhul, Wadi el-Mughara, Israel, 80-120 kya

Homo sapiens Skhul 5 (2501.1rp37-1) by RLA Archaeology on Sketchfab
Wajak, East Java, Indonesia, ~28-38 kya

Homo sapiens (Wadjak 1) (1979rp11) by RLA Archaeology on Sketchfab
Cro-Magnon, Les Eyzies, Dordogne, France ~ 27 kya

Homo sapiens (Cro-Magnon I) Skull (1979rp16-1) by RLA Archaeology on Sketchfab
Previous

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Lab : about fossil hominins based on the analysis of qualitative and quantitative empirical data

Lab : about fossil hominins based on the analysis of qualitative and quantitative empirical data

lab Report (Result)

lab Report (Result). lab Report (Result). Lab Report will be on exercises 5.13, 5.14, 5,15, and 5.16. Below there word document consisting of the pictures of my results from each exercise followed by a paragraph explaining those results.
There are 4 pictures of my results for each exercise write one paragraph that explains the results for each picture.
Below I have attached pdf and power point to help writer the results.

lab Report (Result)

lab Report (Result)

easy assigment

easy assigment. easy assigment. PLEASE ONLY USING DRWINGS IN A PAPER, NO SENTENCES OR PARAGRAPHS JUST LABELING PLACES!!!!
Part 1
Draw, label, caption and upload a diagram that shows how an individual’s genotype is expressed in its phenotype. Include the following:
– The initiation of transcription including these regulatory proteins: one activator, one enhancer, one DNA bending protein, one promoter, one gene, RNA polymerase
– The full process of transcription
– mRNA processing

easy assigment

easy assigment

discussion board replies

discussion board replies. discussion board replies. In Your replies, you will analyze the posts of any two of your classmates describe the risks and advantages of the procedures they wrote about.Your replies should be least 200 words long each. You are encouraged to use at least 2 peer-reviewed scientific sources and review articles in composing your essay. You are to use the AMA writing format.
I will attach the file of the two replies down below

discussion board replies

discussion board replies

Mystery of the Black Death worksheet

Mystery of the Black Death worksheet. 1. The movie was about the Black Death. What is the real name for the disease? 2. What organism was the carrier of the disease, its bite giving you the virus? 3. What was the name of the mutation discussed? 4. What does one need to be completely immune to the Black Death (As far as the mutation goes?) 5. To what modern disease does the mutation discussed give its resistance or immunity to? 6. What percent of people were killed by the plague in europe? 7. If you got the disease, what were the chances you’d survive? 8. How does the plague “Out whit” the immune system? 9. What percent of native Africans

Mystery of the Black Death worksheet